UPLC and UHPLC

Introduction, Principle and Advantages Ultra Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography, generally referenced...

Introduction, Principle and Advantages

Ultra Performance Liquid Chromatography and Ultra High Performance Liquid Chromatography, generally referenced together as UPLC or UHPLC, represent an evolution of conventional HPLC that employs columns packed with sub-two-micrometre particles operated at substantially higher system pressures, frequently exceeding one thousand bar. The use of smaller particles increases theoretical plate number per unit column length in accordance with van Deemter theory, thereby delivering markedly improved chromatographic resolution, shorter analysis times, and reduced solvent consumption relative to conventional HPLC employing larger particle stationary phases. These advantages have made UPLC and UHPLC the preferred platform for high-throughput pharmaceutical and bioanalytical laboratories seeking to increase sample throughput without compromising analytical quality, and the technique is now routinely coupled with tandem mass spectrometric detection in modern bioanalytical workflows.